Междисциплинарный центр фундаментальных исследований и лаборатория перспективных исследований мембранных белков представляют Вашему вниманию открытую лекцию по сверхразрешающей микроскопии.
21 ноября (четверг), 17:05, 115 КПМ
Dr. Klaus Weisshart
Carl Zeiss Microscopy GmbH, BioSciences Division Carl Zeiss Microscopy GmbH, BioSciences Division
Super-resolution microscopy in 3D – Photoactivated Localization and Structured Illumination Microscopy to study cellular structures and dynamics
Resolving ultrafine details of subcellular structures is key to understand the organization and functioning of cellular networks. Recent advances in far-field fluorescence microscopy provide the necessary tools to analyze these structures with resolutions well below the classical diffraction limit in all three-dimensions. These technical advances went hand in hand with improved versions of photo-switchable fluorophores that allowed to push resolution limits further down. The wide spectrum of suitable dyes along with the high contrast achieved endow these fluorescence based superresolution (SR) techniques with the power to study the complexity of sub-cellular organelles and the relation of their constituting components down to the molecular level and under physiological conditions. They provide us in this way with a far better understanding of the assembly of macromolecular complexes and their functions within a cell than has been possible before employing conventional imaging methods. Here we will give an overview of the technical state-of-the art of two of these technologies, Structured Illumination Microscopy (SIM) and Photoactivated Localization Microscopy (PALM), and provide typical application examples in this exciting field.