Laboratory for Advanced Studies of Membrane Proteins

The lab team is focused on structural and biophysical studies of membrane proteins, such as ion channels, transporters, photoactive proteins, G protein-coupled receptors (GPCRs), transmembrane enzymes and their complexes.

The lab team is focused on structural and biophysical studies of membrane proteins, such as ion channels, transporters, photoactive proteins, G protein-coupled receptors (GPCRs), transmembrane enzymes and their complexes. Membrane proteins play a crucial role in many physiological processes mainly involved in signal transduction and membrane transport. Their dysfunction leads to many severe health disorders such as various types of cancer, chronic inflammations, cardiovascular diseases, neurodegenerative disorders. High resolution structures of these proteins are of great importance for understanding underlying mechanisms of these diseasesand to develop rational approaches to cure them. Despite the high importance there is still no structural information for a majority of membrane proteins existing, mainly because they are difficult to handle due to their amphiphilic nature and low stability. In our lab we established the infrastructure to purify and stabilize proteins from heterologous expression systems, such as E. coli, cell free and baculovirus expression systems. A variety of biophysical methods is available for structural and spectroscopic investigations of these protein systems. One of the core methods is the X-ray crystallography of membrane proteins. For this purpose we have setup a unique equipment for high throughput in meso crystallization, crystal imaging and crystallization process investigations. A Rigaku X-Ray HighFlux HomeLab is an important facility in use to characterize the quality of crystals and of proteins in solution. Advantages of modern fluorescence microscopy methods (including CLSM with Spectral Imaging, TIRFM, FCS, FCCS, 2PFM, FLIM, FRAP, SIM, PALM, direct STORM) realized in the Light Nanoscope facility is utilized for investigations of the dynamic properties of proteins and their behavior in a cellular environment. Protein targets can be further characterized with Brucker Ultraflextreme Mass Spectrometry, analytical HPLC system and differential fluorimetry to study protein thermostability.

Georg Büldt

Head of laboratory

Education
1969 Diploma in Physics at the Technical University Berlin 

1972 Dissertation (Dr. rer. nat.) in Physical Chemistry, University of Mainz
Professional experience
1969 Diploma in Physics at the Technical University Berlin

1972 Dissertation (Dr. rer. nat.) in Physical Chemistry, University of Mainz

1978 Habilitation in Biophysics at the Biocenter of the University of Basel, Switzerland 

1982 - 1992 C2-Professor at the Physics Department of the Free University Berlin 

1993 - 2008 C4-Professor for Physical Biology at the Heinrich-Heine-University Düsseldorf, Germany 

1993 - 2011 Director at the Institute of Complex Systems (ICS) of the Research Center Jülich, ICS-5: Molecular Biophysics 

1996 Offer of a chair in Biophysics at the Johann-Wolfgang-Goethe University Frankfurt, which was rejected 

2004 - 2010 Speaker of the Virtual Institute for Structural Biology 

2011 - 2013 Founding Director of the Laboratory of Advanced Studies of Membrane Proteins at Moscow Institute of Physics and Technology (MIPT) 

Since 2014 Continuation as a Director of this Laboratory
Academic Interests
Systems: Membrane proteins and proteins of signal amplification cascades; mechanisms for the transport of protons and signals across membranes; protein folding. 

Methods: X-ray, neutron and electron diffraction on 2D and 3D protein crystals, crystal structures of intermediate states in the working cycle of proteins; micro-spectroscopy on single crystals trapped in intermediate states (visible, infrared); inelastic neutron scattering on equilibrium fluctuations in proteins, single molecule fluorescence spectroscopy.
Publications

2015, NATURE STRUCTURAL & MOLECULAR BIOLOGY (11.633)

2014, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (7.232)

2013, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY (2.803)

2012, NEW JOURNAL OF PHYSICS (3.671)

2011, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (9.809)

2011, JOURNAL OF MOLECULAR BIOLOGY (3.959)

2010, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (7.232)

2008, JOURNAL OF SURFACE INVESTIGATION-X-RAY SYNCHROTRON AND NEUTRON TECHNIQUES (0.359)

Contacts
Phone +49 2461 61-2030
Email g.bueldt@fz-juelich.de

Valentin Borshchevskiy

Deputy head of laboratory, senior researcher

Education
ScD in physics and mathematics
Publications

2015, NATURE STRUCTURAL & MOLECULAR BIOLOGY (11.633)

2014, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (7.232)

2013, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY (2.803)

2013, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (9.809)

2013, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (9.809)

2011, JOURNAL OF MOLECULAR BIOLOGY (3.959)

2010, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (7.232)

2008, JOURNAL OF SURFACE INVESTIGATION-X-RAY SYNCHROTRON AND NEUTRON TECHNIQUES (0.359)

Margarita Zaytseva

Engineer

Andrey Bogorodskiy

Junior researcher

Alexey Alexeev

Junior researcher

Kirill Kovalev

Junior researcher

Egor Zinovyev

Junior researcher

Yuri Rizhikov

Junior researcher

Alexey Vlasov

Junior researcher

Egor Maryin

Technician

Yuri Kovalev

Engineer

Education
PhD in physics and mathematics

Alexander Kuklin

Senior researcher

Education
PhD in physics and mathematics

Alexander Ivankov

Senior researcher

Education
PhD in physics and mathematics

Andrey Rogachev

Senior researcher

Education
PhD in physics and mathematics

Tatyana Murugova

Senior researcher

Education
PhD in physics and mathematics

Dmitry Solovyov

Senior researcher

Education
PhD in physics and mathematics

Nikolay Ilyinskiy

Senior researcher

Education

PhD in physics and mathematics

Ivan Okhrimenko

Researcher

Nina Malyar

Junior researcher

Ivan Maslov

Technician

Maria Balashoyu

Senior researcher

Education
PhD in physics and mathematics

2015

NATURE STRUCTURAL & MOLECULAR BIOLOGY (11.633)

Crystal structure of a light-driven sodium pump

Gushchin, I, Shevchenko, V, Polovinkin, V, Kovalev, K, Alekseev, A, Round, E, Borshchevskiy, V, Balandin, T, Popov, A, Gensch, T, Fahlke, C, Bamann, C, Willbold, D, Buldt, G, Bamberg, E, Gordeliy, V

Moscow Institute of Physics and Technology

CRYSTAL GROWTH & DESIGN (4.558)

Nucleation and Growth of Membrane Protein Crystals in Meso - A Fluorescence Microscopy Study

Bogorodskiy A., Frolov F., Mishin A., Round E., Polovinkin V., Cherezov V., Gordeliy V., Büldt G., Gensch T., Borshchevskiy V.

Moscow Institute of Physics and Technology

PLOS ONE (3.534)

An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin

Bratanov, D, Balandin, T, Round, E, Shevchenko, V, Gushchin, I, Polovinkin, V, Borshchevskiy, V, Gordeliy, V

Moscow Institute of Physics and Technology

PLOS ONE (3.534)

Structural and Functional Investigation of Flavin Binding Center of the NqrC Subunit of Sodium-Translocating NADH: Quinone Oxidoreductase from Vibrio harveyi

Borshchevskiy, V, Round, E, Bertsova, Y, Polovinkin, V, Gushchin, I, Ishchenko, A, Kovalev, K, Mishin, A, Kachalova, G, Popov, A, Bogachev, A, Gordeliy, V

Moscow Institute of Physics and Technology

2014

PLOS ONE (3.534)

Crystal Structure of Escherichia coli-Expressed Haloarcula marismortui Bacteriorhodopsin I in the Trimeric Form

Shevchenko, V, Gushchin, I, Polovinkin, V, Round, E, Borshchevskiy, V, Utrobin, P, Popov, A, Balandin, T, Buldt, G, Gordeliy, V

Moscow Institute of Physics and Technology

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (7.232)

Low-dose X-ray radiation induces structural alterations in proteins

Borshchevskiy, V, Round, E, Erofeev, I, Weik, M, Ishchenko, A, Gushchin, I, Mishin, A, Willbold, D, Buldt, G, Gordeliy, V

Moscow Institute of Physics and Technology

CHEMBIOCHEM (3.06)

Nanosecond Dynamics of Calmodulin and RibosomeBound Nascent Chains Studied by Time- Resolved Fluorescence Anisotropy

Lamprou, P, Kempe, D, Katranidis, A, Buldt, G, Fitter, J

Moscow Institute of Physics and Technology

JOURNAL OF MEMBRANE BIOLOGY (2.174)

Nanoparticle Surface-Enhanced Raman Scattering of Bacteriorhodopsin Stabilized by Amphipol A8-35

Polovinkin, V, Balandin, T, Volkov, O, Round, E, Borshchevskiy, V, Utrobin, P, von Stetten, D, Royant, A, Willbold, D, Arzumanyan, G, Chupin, V, Popot, JL, Gordeliy, V

Moscow Institute of Physics and Technology

JOURNAL OF MEMBRANE BIOLOGY (2.174)

High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase

Polovinkin, V, Gushchin, I, Sintsov, M, Round, E, Balandin, T, Chervakov, P, Schevchenko, V, Utrobin, P, Popov, A, Borshchevskiy, V, Mishin, A, Kuklin, A, Willbold, D, Chupin, V, Popot, JL, Gordeliy, V

Moscow Institute of Physics and Technology

NATURE COMMUNICATIONS (10.742)

X-ray structure of a CDP-alcohol phosphatidyltransferase membrane enzyme and insights into its catalytic mechanism

Nogly, P, Gushchin, I, Remeeva, A, Esteves, AM, Borges, N, Ma, P, Ishchenko, A, Grudinin, S, Round, E, Moraes, I, Borshchevskiy, V, Santos, H, Gordeliy, V, Archer, M

Moscow Institute of Physics and Technology

2013

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY (2.803)

Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer

Ishchenko, A, Round, E, Borshchevskiy, V, Grudinin, S, Gushchin, I, Klare, JP, Balandin, T, Remeeva, A, Engelhard, M, Buldt, G, Gordeliy, V

Moscow Institute of Physics and Technology

NATURE (42.351)

STRUCTURAL BIOLOGY Active arrestin proteins crystallized

Borshchevskiy, V, Buldt, G

Moscow Institute of Physics and Technology

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (9.809)

Structural insights into the proton pumping by unusual proteorhodopsin from nonmarine bacteria (vol 110, pg 12631, 2013)

Gushchin, I, Chervakov, P, Kuzmichev, P, Popov, AN, Round, E, Borshchevskiy, V, Ishchenko, A, Petrovskaya, L, Chupin, V, Dolgikh, DA, Arseniev, AS, Kirpichnikov, M, Gordeliy, V

Moscow Institute of Physics and Technology

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (9.809)

Structural insights into the proton pumping by unusual proteorhodopsin from nonmarine bacteria

Gushchin, I, Chervakov, P, Kuzmichev, P, Popov, AN, Round, E, Borshchevskiy, V, Ishchenko, A, Petrovskaya, L, Chupin, V, Dolgikh, DA, Arseniev, AA, Kirpichnikov, M, Gordeliy, V

Moscow Institute of Physics and Technology

2012

JOURNAL OF MOLECULAR BIOLOGY (3.959)

Crystal Structure of p44, a Constitutively Active Splice Variant of Visual Arrestin

Granzin, J, Cousin, A, Weirauch, M, Schlesinger, R, Buldt, G, Batra-Safferling, R

Moscow Institute of Physics and Technology

NEW JOURNAL OF PHYSICS (3.671)

Soft x-ray tomoholography

Guehrs, E, Stadler, AM, Flewett, S, Frommel, S, Geilhufe, J, Pfau, B, Rander, T, Schaffert, S, Buldt, G, Eisebitt, S

Moscow Institute of Physics and Technology

2011

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (9.809)

Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process

Kirchberg, K, Kim, TY, Moller, M, Skegro, D, Raju, GD, Granzin, J, Buldt, G, Schlesinger, R, Alexiev, U

Moscow Institute of Physics and Technology

JOURNAL OF MOLECULAR BIOLOGY (3.959)

Active State of Sensory Rhodopsin II: Structural Determinants for Signal Transfer and Proton Pumping

Gushchin, I, Reshetnyak, A, Borshchevskiy, V, Ishchenko, A, Round, E, Grudinin, S, Engelhard, M, Buldt, G, Gordeliy, V

Moscow Institute of Physics and Technology

JOURNAL OF MOLECULAR BIOLOGY (3.959)

X-ray-Radiation-Induced Changes in Bacteriorhodopsin Structure

Borshchevskiy, VI, Round, ES, Popov, AN, Buldt, G, Gordeliy, VI

Moscow Institute of Physics and Technology

2010

JOURNAL OF CRYSTAL GROWTH (1.693)

Isoprenoid-chained lipid beta-XylOC(16+4)-A novel molecule for in meso membrane protein crystallization

Borshchevskiy, V, Moiseeva, E, Kuklin, A, Buldt, G, Hato, M, Gordeliy, V

Moscow Institute of Physics and Technology

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (7.232)

Overcoming merohedral twinning in crystals of bacteriorhodopsin grown in lipidic mesophase

Borshchevskiy, V, Efremov, R, Moiseeva, E, Buldt, G, Gordeliy, V

Moscow Institute of Physics and Technology

2009

JOURNAL OF SURFACE INVESTIGATION-X-RAY SYNCHROTRON AND NEUTRON TECHNIQUES (0.359)

Comparative analysis of the quality of membrane protein bacteriorhodopsin crystals during crystallization in octylglucoside and octylthioglucoside

Moiseeva, ES, Reshetnyak, AB, Borshchevskiy, VI, Baeken, C, Buldt, G, Gordeliy, VI

Moscow Institute of Physics and Technology

2008

JOURNAL OF SURFACE INVESTIGATION-X-RAY SYNCHROTRON AND NEUTRON TECHNIQUES (0.359)

Comparative analysis of sensory rhodopsin II structures in complex with a transducer and without it

Reshetnyak A.B., Borshchevskiy V.I., Klare J., Moiseeva E.S., Engelhardt M., Buldt G., Gordeliy V.I.

Moscow Institute of Physics and Technology

2006

BIOPHYSICAL JOURNAL (3.832)

Time-resolved microspectroscopy on a single crystal of bacteriorhodopsin reveals lattice-induced differences in the photocycle kinetics

Efremov, R, Gordeliy, VI, Heberle, J, Buldt, G

Moscow Institute of Physics and Technology

NATURE (42.351)

Development of the signal in sensory rhodopsin and its transfer to the cognate transducer

Moukhametzianov, R, Klare, JP, Efremov, R, Baeken, C, Goppner, A, Labahn, J, Engelhard, M, Buldt, G, Gordeliy VI

Moscow Institute of Physics and Technology

2005

FEBS LETTERS (3.341)

Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli

Mironova, OS, Efremova, RG, Person, B, Heberle, J, Budyak, IL, Buldt, G, Schlesinger, R

Moscow Institute of Physics and Technology

BIOPHYSICAL JOURNAL (3.832)

Water molecules and hydrogen-bonded networks in bacteriorhodopsin - Molecular dynamics simulations of the ground state and the M-intermediate

Grudinin, S, Buldt, G, Gordeliy V, Baumgaertner, A

Moscow Institute of Physics and Technology

JOURNAL OF CRYSTAL GROWTH (1.693)

Crystallization of F1F0-ATP synthase from Chloroflexus aurantiacus

Kiselyova, OI, Shiryaeva, GN, Efremov, RG, Gordeliy, VI, Yaminsky, IV, Yanyushin, MF, Buldt, G, Yaguzhinsky, LS

Moscow Institute of Physics and Technology

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (3.096)

Particles of liquid-crystalline dispersions formed by (nucleic acid-rare earth element) complexes as a potential platform for neutron capture therapy

Yevdokimov, YM, Salyanov, VI, Kondrashina, OV, Borshevsky, VI, Semenov, SV, Gasanov, AA, Reshetov, IV, Kuznetsov, VD, Nikiforov, VN, Akulinichev, SV, Mordovskoi, MV, Potashev, SI, Skorkin, VM

Moscow Institute of Physics and Technology

2004

BIOPHYSICAL JOURNAL (3.832)

Physical detwinning of hemihedrally twinned hexagonal crystals of bacteriorhodopsin

Efremov, R, Moukhametzianov, R, Buldt, G, Gordeliy V

Moscow Institute of Physics and Technology

2002

NATURE (42.351)

Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex

Gordeliy, VI, Labahn, J, Moukhametzianov, R, Efremov, R, Granzin, J, Schlesinger, R, Buldt, G, Savopol, T, Scheidig, AJ, Klare, JP, Engelhard, M

Moscow Institute of Physics and Technology

FEBS JOURNAL (3.986)

Structure of the light-driven sodium pump KR2 and its implications for optogenetics

Gushchin I., Shevchenko V., Polovinkin V., Borshchevskiy V., Buslaev P., Bamberg E., Gordeliy V.

Moscow Institute of Physics and Technology

2016

Journal of the American Chemical Society (13.038)

Highly Sensitive Coherent Anti-Stokes Raman Scattering Imaging of Protein Crystals

Grigory Arzumanyan, Nelya Viktorovna Doroshkevich, Kahramon Mamatkulov, Sergey Shashkov, Egor Vladimirovich Zinovev, Alexey Vlasov, Ekaterina Round, and Valentin Gordeliy

Moscow Institute of Physics and Technology

Nature Communications (11.329)

Revealing the mechanism of passive transport in lipid bilayers via phonon-mediated nanometre-scale density fluctuations

Zhernenkov M, Bolmatov D, Soloviov D, Zhernenkov K, Toperverg BP, Cunsolo A, Bosak A, Cai YQ

Moscow Institute of Physics and Technology

Nanoscale research letters (2.584)

SAXS Combined with UV-vis Spectroscopy and QELS: Accurate Characterization of Silver Sols Synthesized in Polymer Matrices

Bulavin, L., Kutsevol, N., Chumachenko, V., Soloviov, D., Kuklin, A., & Marynin, A.

Moscow Institute of Physics and Technology

Biochimica et Biophysica Acta Bioenergetics (5.158)

On the existence of two states of OXPHOS system supercomplex in heart mitochondria

Byvshev, I. M., Vangeli, I. M., Murugova, T. N., Ivankov, O. O., Kuklin, A. I., Popov, V. I., ... & Yaguzhinsky, L. S.

Moscow Institute of Physics and Technology

2017

Science (37.205)

Structural insights into ion conduction by channelrhodopsin 2

Volkov, O., Kovalev, K., Polovinkin, V., Borshchevskiy, V., Bamann, C., Astashkin, R., Marin, E., Popov, A., Balandin, T., Willbold, D., Büldt, G., Bamberg, E. & Gordeliy, V.

Moscow Institute of Physics and Technology

PLoS One

Green fluorescent genetically encoded calcium indicator based on calmodulin/M13-peptide from fungi

Barykina, N. V, Subach, O. M., Piatkevich, K. D., Jung, E. E., Malyshev, A. Y., Smirnov, I. V, Bogorodskiy, A. O., Borshchevskiy, V. I., Varizhuk, A. M., Pozmogova, G. E., Boyden, E. S., Anokhin, K. V, Enikolopov, G. N. & Subach, F. V.

Moscow Institute of Physics and Technology

Science (37.205)

Mechanism of transmembrane signaling by sensor histidine kinases

Gushchin, I., Melnikov, I., Polovinkin, V., Ishchenko, A., Yuzhakova, A., Buslaev, P., Bourenkov, G., Grudinin, S., Round, E., Balandin, T., Borshchevskiy, V., Willbold, D., Leonard, G., Büldt, G., Popov, A. & Gordeliy, V.

Moscow Institute of Physics and Technology

Crystal Growth and Design

Chemically Stable Lipids for Membrane Protein Crystallization

Ishchenko, A., Peng, L., Zinovev, E., Vlasov, A., Lee, S. C., Kuklin, A., Mishin, A., Borshchevskiy, V., Zhang, Q. & Cherezov, V.

Moscow Institute of Physics and Technology

Science Advances

Fast iodide-SAD phasing for high-throughput membrane protein structure determination

Melnikov, I., Polovinkin, V., Kovalev, K., Gushchin, I., Shevtsov, M., Shevchenko, V., Mishin, A., Alekseev, A., Rodriguez-Valera, F., Borshchevskiy, V., Cherezov, V., Leonard, G. A., Gordeliy, V. & Popov, A.

Moscow Institute of Physics and Technology

Science Advances

Inward H + pump xenorhodopsin: Mechanism and alternative optogenetic approach

Shevchenko, V., Mager, T., Kovalev, K., Polovinkin, V., Alekseev, A., Juettner, J., Chizhov, I., Bamann, C., Vavourakis, C., Ghai, R., Gushchin, I., Borshchevskiy, V., Rogachev, A., Melnikov, I., Popov, A., Balandin, T., Rodriguez-Valera, F., Manstein, D. J., Bueldt, G., Bamberg, E. & Gordeliy, V.

Moscow Institute of Physics and Technology

Scientific Reports

New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex

shchenko, A., Round, E., Borshchevskiy, V., Grudinin, S., Gushchin, I., Klare, J. P., Remeeva, A., Polovinkin, V., Utrobin, P., Balandin, T., Engelhard, M., Büldt, G. & Gordeliy, V.

Moscow Institute of Physics and Technology

Crystal Growth and Design

Integral Membrane Proteins Can Be Crystallized Directly from Nanodiscs

Nikolaev, M., Round, E., Gushchin, I., Polovinkin, V., Balandin, T., Kuzmichev, P., Shevchenko, V., Borshchevskiy, V., Kuklin, A., Round, A., Bernhard, F., Willbold, D., Büldt, G. & Gordeliy, V.

Moscow Institute of Physics and Technology

Institut de Biologie Structurale Jean-Pierre Ebel (IBS)

The French Institute for Research in Computer Science and Automation (Inria)

The Scripps Research Institute

The Institutes of the Research Center Juelich

Lomonosov Moscow State University

The Institute of Bioorganic Chemistry (RAS)

Joint Institute for Nuclear Research

European Synchrotron Radiation Facility (ESRF)

European Molecular Biology Laboratory (EMBL)

The European Molecular Biology Laboratory

MRC Laboratory of Molecular Biology