© 2001-2018 Moscow Institute of Physics and Technology
Laboratory for Advanced Studies of Membrane Proteins
In our lab we established the infrastructure to purify and stabilize proteins from heterologous expression systems, such as E. coli, cell free and baculovirus expression systems. A variety of biophysical methods are available for structural and spectroscopic investigations of these protein systems. One of the core methods is the X-ray crystallography of membrane proteins. For this purpose we have setup a unique equipment for high throughput in meso crystallization, crystal imaging and crystallization process investigations. A Rigaku X-Ray HighFlux HomeLab is an important facility in use to characterize the quality of crystals and of proteins in solution. Advantages of modern fluorescence microscopy methods (including CLSM with Spectral Imaging, TIRFM, FCS, FCCS, 2PFM, FLIM, FRAP, SIM, PALM, direct STORM) realized in the Light Nanoscope facility is utilized for investigations of the dynamic properties of proteins and their behavior in a cellular environment. Protein targets can be further characterized with Brucker Ultraflextreme Mass Spectrometry, analytical HPLC system and differential fluorimetry to study protein thermostability.
Vice-Head: Dr. Valentin Borshchevskiy